HIGH-LEVEL PRODUCTION OF DIACETYL IN A METABOLICALLY ENGINEERED LACTIC ACID BACTERIUM

USPTO Patent Application US 2018/0282827 A1
Application Date: Oct. 4th, 2018

ABSTRACT

The present invention provides a genetically modified lactic acid bacterium capable of producing diacetyl under aerobic conditions. Additionally the invention provides a method for producing diacetyl using the genetically modified...
Application Number:
15/766,461
International Classification:
C12R 1/225 (20060101); A23L 27/24 (20060101); C12P 7/26 (20060101)
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CLAIMS

What is claimed is:

1. A genetically modified lactic acid bacterium for production of diacetyl, wherein the genome of said lactic acid bacterium is deleted for genes or lacks genes encoding polypeptides having an enzymatic activity of: a. lactate dehydrogenase (E.C.1.1.1.27 or E.C.1.1.1.28)b. α-acetolactate decarboxylase (E.C. 4.1.1.5)c. phosphotransacetylase (E.C.2.3.1.8) andd. NADH oxidase (E.C. 1.6.3.4); andwherein...

DESCRIPTION

FIELD OF THE INVENTION

The present invention provides a genetically modified lactic acid bacterium capable of producing diacetyl under aerobic conditions. Additionally the invention provides a method for producing diacetyl using the genetically modified lactic acid bacterium under aerobic conditions in the presence of a source of iron-containing porphyrin and a metal ion selected...
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