What is claimed is:
1. A method for the isolation of bacteriophage-insensitive mutants from a Streptococcus thermophilus parent strain comprising:
a. inactivating the CRISPR resistance mechanism of the parent strain;b. exposing the parent strain obtained in step a) to a bacteriophage;c. isolating bacteriophage insensitive mutants;d. comparing the CRISPR loci of the parent strain with the...
FIELD OF THE INVENTION
The present invention relates to a method for the construction of bacteriophage-insensitive Streptococcus thermophilus mutants, whereby a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system is attenuated, leading to phage resistance being conferred by an alternative mechanism.