PHAGE RESISTANT LACTIC ACID BACTERIA

USPTO Patent Application US 2017/0196233 A1
Application Date: Jul. 13rd, 2017

ABSTRACT

The present invention provides a method for the construction of a bacteriophage-insensitive mutant of a Streptococcus thermophilus parent strain whereby the bacteriophage insensitivity is not based on the CRISPR resistance...
Application Number:
15/327,661
International Classification:
A23C 9/123 (20060101); A23C 19/032 (20060101); C12N 15/90 (20060101); C12N 15/74 (20060101); C12N 15/113 (20060101)
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CLAIMS

What is claimed is:

1. A method for the isolation of bacteriophage-insensitive mutants from a Streptococcus thermophilus parent strain comprising: a. inactivating the CRISPR resistance mechanism of the parent strain;b. exposing the parent strain obtained in step a) to a bacteriophage;c. isolating bacteriophage insensitive mutants;d. comparing the CRISPR loci of the parent strain with the...

DESCRIPTION

FIELD OF THE INVENTION

The present invention relates to a method for the construction of bacteriophage-insensitive Streptococcus thermophilus mutants, whereby a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system is attenuated, leading to phage resistance being conferred by an alternative mechanism.
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