METHODS FOR DISCRIMINATING INTERSPECIFIC RELATIONSHIPS AND INTRASPECIFIC RELATIONSHIPS OF LACTIC ACID BACTERIA OF LACTOBACILLUS CASEI GROUP

USPTO Patent Application US 2017/0058327 A1
Application Date: Mar. 2nd, 2017

ABSTRACT

Degenerate primers for amplifying fragments of the nucleotide sequences of mutL and dnaJ genes of a lactic acid bacterium of Lactobacillus casei group are provided. Methods and kits for discriminating...
Application Number:
14/959,587
International Classification:
C12Q 1/68 (20060101)
No Pictures Available

CLAIMS

What is claimed is:

1. A degenerate primer pair comprising: a first primer mixture comprising the nucleotide sequences shown as 5′-ATTGAYCARCTATCKGCMAC-3′ (SEQ ID NO: 1), wherein Y=C or T; R=A or G; K=G or T; and M=A or C, anda second primer mixture comprising the nucleotide sequences shown as 5′-GAGCCRTAACCGGCAATMA-3′ (SEQ ID NO: 2), wherein...

DESCRIPTION

FIELD OF THE APPLICATION

The present application relates to a method for identifying the related taxa of lactic acid bacteria of Lactobacillus casei group by using a primer pair to amplify the sequence of a specific targeted gene, and then sequencing and comparing the amplified sequence.
Back to Top